Transfer the cell suspension to a centrifuge tube, add 10 ml of the medium specified in the catalog, centrifuge the mixture at 1000 rpm for 3 min, and discard the supernatant. Although every ampoule has a groove around the neck for easy cutting off, a precaution may be required to open the ampoule because of its hard structure. Sterilize the entire surface of the ampoule with gauze moist with 70% ethanol or cationic detergent sterilizer, and cut the neck off while wrapped in sterilized gauze. It is safe to thaw them one by one, even if it takes longer.Īll operations should be performed under aseptic conditions. Use safety gloves and a face shield to avoid injury from possible explosion of the ampoule. Take out the ampoule from the styrene foam package and thaw the content within 2 min by shaking in warm (not higher than 37☌.) water. It is recommended to preserve them in vapor phase of liquid nitrogen tank. If cryotube or glass ampoule has been stored directly in liquid nitrogen (liquid phase), there is a risk of exploding imperfectly sealed cryotube or glass.
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It will result in rapid loss of viability.ĭo not immerse in liquid nitrogen directly!!! If the cell lines would be stored in a freezer, they should be kept below –80 ☌, and should not be stored longer than 30 days. *Please read the important technical information that follows before handling JCRB-supplied cell lines*.Ĭell lines should be stored in liquid nitrogen vapor or cultured immediately after arrival.